University science and creative park - additions in 2020
Real-time PCR (Polymerase chain reaction, PCR)
Polymerase chain reaction (PCR) is a method and technique for making millions to billions of copies of a specific sample of DNA (deoxyribonucleic acid) - a carrier of genetic information.
The “AriaMx real-time PCR” instrument (fy Agilent) is a fully integrated qPCR system for data amplification, detection and analysis. The modular design of the system combines a modern thermal cycler, an advanced optical system and data analysis software. Its advantage is speed, dexterity and accuracy.
Microplate counter Multiskan Sky
The Multiskan Sky microplate spectrophotometer combines the advantages of standard microplate counters, cuvette spectrophotometers and photometers designed for photometric analysis of DNA, RNA and proteins in microvolumes. Analyzes can be performed in the visible range of radiation, primarily for the purpose of determining the color components of various solutions, but also enzyme activities and selected chemicals using chromogenic reactions. In addition, if special microtiter plates are used, this device can also be used to determine specific components in the UV range.
The device is used to evaluate the activities of enzymes belonging to the classes of oxidases (lactase, peroxidase, glucose oxidase) and hydrolases (amylase, protease, cellulase, sucrose, lipase, neuraminidase), but also to determine the concentration of carbohydrates, amino acids, proteins, polyphenols and antioxidants. present in plant extracts.
Nuclear magnetic resonance spectrometer Pulsar (Oxford Instruments)
The Pulsar benchtop NMR instrument (Oxford Instruments) is a new category for a high-resolution method for the identification of organic compounds, bioorganic derivatives, drugs, nucleic acid building blocks, proteins and enzymes. In Slovakia, it is the first of its kind purchased and installed for teaching purposes.
The advantage of a benchtop NMR instrument is especially its affordability compared to hall devices operating at higher frequencies of excitation radiation and higher magnetic fields. The operation does not require a specialized team of workers and its operation is easy. This practice technique, which is often necessary in practice, is now also available to students who are able to undergo products from laboratory exercises or final theses almost immediately.
EPR (Electron Paramagnetic Resonance)
EPR (Electron Paramagnetic Resonance) is a spectroscopic method and technique that detects free (unpaired) electrons found in organic radicals and transition metal complexes resp. lanthanides. It also detects defects in materials.
Bench-top ESR5000 EPR spectrometer (fy Bruker) is a powerful instrument with high sensitivity and reliability. It is used to detect electron levels in paramagnetic complex compounds.
